| | Optical Design | | Archive ! (02/2005) |
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| plate::vision - the Core of our HTS Systems |  Systems Integration
Assay Development
New Detection Mode
Optical Design
Applications
Technical Data |
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| The enhanced modular design of plate::vision coupled to an open automation architecture allowed Zeiss to introduce a flexible high-throughput platform based on the plate::vision reader. Thus, the Zeiss plate::vision product line is the perfect equipment to address current and future screening needs with a broad range of instruments. |
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| Optimized Detection through Minilens Arrays | |
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| The plate::vision is the first commercially available multimode detection system measuring 96 channels simultaneously and delivering high performance data in microplate formats up to 1536 wells. | |
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The optical design, which eliminates most of the well-known drawbacks of other readers, features various mini-lens arrays (MLA) to choose from. So the best assay results are ensured for each detection technique, independent of the well format.
The mini-lens arrays have 96 miniaturized microscopic objectives for parallel detection. The MLA for fluorescence provides a quasi-confocal illumination of the wells for background reduction (see graphic). For absorbance the beam path in the wells is very narrow to reduce the meniscus effect.
The Zeiss MLAs are designed to match the special requirements of different detection techniques, with the aim to optimize sensitivity, dynamic range, signal-to-noise ratio and crosstalk for each detection mode.
When it comes to cell-based assay screening, the special "bottom-sensitive fluorescence" setup of the plate::vision can perform an automated x, y and z microscan of the well for better discrimination of hits. | |
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