ZOYC@University of Wuerzburg

News on Life Cell Imaging

Pushing the limits of light microscopy

The department of Biotechnology and Biophysics in Würzburg together with ZEISS, are proud to host a workshop about new technologies in microscopy.
Life cell and life tissue fluorescence imaging became a valuable tool in modern research applications in the last decade. Many applications require dedicated solutions and technologies to guarantee optimal and reliable results. Furthermore, image analysis provides the necessary tools to extract quantitative data even from complex and large data sets. In this workshop we will introduce theoretical background information as well as extensive hands-on sessions to better understand and use different systems and software solutions. See the technologies in action and learn how your work can benefit from.

February 28 - March 1, 2023

Biotechnology and Biophysics
University of Würzburg
Building B1, Hubland Sued (Biocenter)
Am Hubland
97074 Würzburg

February 28, 2023 \| 9 AM - 5.30 PM
9 AM	Registration & Coffee
9.30 AM	Welcome	Prof. Dr. Markus Sauer, Department of Biotechnology and Biophysics Biocenter, University of Wuerzburg
9.45 AM	From pure insights to understanding: ZEISS LSM 900 – Airyscan 2 – Multiplexing	Dr. Jan-Erik Heil, ZEISS Microscopy
10.15 AM	The new era of super-resolution microscopy – ZEISS Elyra 7 with Lattice SIM²	Dr. Maximilian Voll, ZEISS Microscopy
10.45 AM	Coffee Break
11 AM	Explore Lattice Lightsheet Microscopy – The evolving technology setting new standards in volumetric live cell imaging	Gert Sonntag, ZEISS Microscopy
11.30 AM	Standard and advanced use of Elyra 7 for super-resolution fluorescence imaging	Prof. Dr. Markus Sauer, Department of Biotechnology and Biophysics Biocenter, University of Wuerzburg
12.15 PM	Lunch Break
1.15 - 5.30 PM	One-On-One Demonstrations We are happy to show you the ZEISS systems and answer your questions in a 60 minutes one-on-one demonstration with your own samples. 1.15 - 2.15 PM 2.15 - 3.15 PM 3.30 - 4.30 PM 4.30 - 5.30 PM
7 PM	Joint dinner with ZEISS Team

March 1, 2023 \| 9 AM - 5.15 PM
9 AM	Coffee
9.30 AM	Powerful solutions for data management and image analysis Part one: Be all ZEN Part two: Push the envelope with Arivis by ZEISS	Dr. Tamara Manuelian, ZEISS Microscopy Dr. Oliver Tress, ZEISS Microscopy
10.30 AM	Coffee Break
10.45 AM	Expansion Microscopy - Lattice SIM	Prof. Dr. Markus Sauer, Department of Biotechnology and Biophysics Biocenter, University of Wuerzburg
11.45 AM	Super-resolution confocal microscopy for translational Bioimaging	Prof. Dr. Katrin Heinze, Rudolf-Virchow-Zentrum - Center for Integrative and Translational Bioimaging, University of Wuerzburg
12.15 PM	Lunch Break
1 - 5.15 PM	One-On-One Demonstrations We are happy to show you the ZEISS systems and answer your questions in a 60 minutes one-on-one demonstration with your own samples. Register for an individual demo slot - sharing with additional participants are welcome. 1.00 - 2.00 PM 2.00 - 3.00 PM 3.15 - 4.15 PM 4.15 - 5.15 PM
5.15 PM	End of Workshop

Live Demonstrations
Stations	Learnings
Station 1 \| ZEISS LSM 900 with Airyscan	Learn how Airyscan technology is applied in gentle imaging We will show you the optimal confocal setup for live cell imaging See the performance increase in resolution with LSM plus Experience up to 90nm resolution with Airyscan Joint Deconvolution (jDCV) See the benefits of Connected Microscopy workflows to keep image data in context
Station 2 \| ZEISS Elyra 7	Learn how superresolution structured illumination microscopy will improve resolution down to 60nm We will introduce and demonstrate different acquisition modalities of Elyra 7 for gentle and fast live cell imaging See how superresolution structured illumination can by applied in high speed up to 255 frames per second
Station 3 \| ZEISS Lattice Lightsheet 7	Learn how the use of Lattice Lightsheet technology enables imaging with subcellular resolution See the use of standard cell culture dishes with glass bottoms on the Lattice Lightsheet 7 We will demonstrate the easy and semi-automatic calibration of the instrument in use You will see how fluorescence imaging of a time series image is performed with low photobleaching and phototoxic effects
Station 4 \| ZEISS ZEN Software	We will introduce and train the basics of post processing and image analysis for fluorescence image data sets Discover how image analysis can be automized and applied on a series of data sets Lean how Artificial Intelligence (AI) methods can be used for reliable segmentation of fluorescence data
Station 5 \| ZEISS arivis Software	Learn how large 3D data sets can be visualized and analyzed Discover powerful tools for the analysis of time lapse data sets including 3D tracking and tracing See how an image analysis pipeline can be created and applied

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