Long-Term Volumetric Imaging of Living Cells @ Lattice Lightsheet 7

Lattice Lightsheet 7 makes light sheet fluorescence microscopy available for live cell imaging at subcellular resolution – while also allowing you to use your standard sample carriers. With this automated, easy-to-use system, volumetric imaging of subcellular structures and dynamics over hours and day with best protection from photo damage becomes available to everyone.
This course will provide the lattice light sheet principle, its advantages for 3D imaging of subcellular dynamics and the skills to acquire lattice lightsheet data from a variety of specimens.

Topics of the course

 

  • System hardware
  • Light path principle
  • Light sheet alignment
  • Stage leveling
  • Incubation, long time experiments
  • Overview scan, volume scan, multiple positions and tiling
  • PS measurement 
  • Lattice Lightsheet processing (Deskew, Coverglass Transformation and Deconvolution)

 

Learning Level

Advanced

 

Prerequisites

Basic knowledge of light microscopy

 

Systems

Lattice Lightsheet 7

 

Software

ZEN (blue edition)

 

Date

September 27 to 28, 2022 
Registration until 2 weeks prior to the training

 

Duration

2 days (2 x 4 hours)

 

Location

Virtual classroom
(Required: reliable internet connection; computer speakers, headphones or a reliable telephone connection; TeamViewer)

 

Language

English

 

Course Fee

Per person: 1080 € (plus VAT)

 

Participants

Max. 3

 

For additional information please send a request to courses .microscopy .de @zeiss .com.

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