Superresolution Microscopy @ Elyra 7

Superresolution microscopy enables insights into structures beyond the classical resolution limit. Since the introduction of Elyra 7, structured illumination microscopy (SIM) has been brought to a new level. Groundbreaking light efficiency empowers gentle superresolution imaging with incredibly high speed: to examine the fastest processes in living samples – in large fields of view, in 3D, over long time periods, and with multiple colors.
Elyra 7 combines Lattice SIM with single molecule localization microscopy (SMLM) for techniques such as PALM, dSTORM and PAINT.
This course will provide the skills to acquire Lattice SIM, PALM, and dSTORM data from specimens and will enable you to perform the appropriate processing. 

Topics of the course

 

Lattice SIM
  • Principle, acquisition and filtering
  • Versatility: SIM Mode, Leap Mode, Apotome Mode, Burst Mode
  • Camera alignment, channel alignment
  • PSF measurement
  • Processing

 

Single-molecule localization microscopy 

  • Basics and applications
  • Comparison: SMLM is more than PALM
  • Multichannel imaging
  • Grouping and working with fiducials
  • Activation strategies
  • 3D PALM

 

Learning Level

Expert

 

Prerequisites

Profound knowledge of laser scanning microscopy

 

Systems

Elyra 7

 

Software

ZEN SR black

 

Date

June 21 to 24, 2022 
Registration until 2 weeks prior to the training

 

Duration

4 days

 

Location

Training and Support Center Jena

 

Language

English

 

Course Fees

Per person: 2880 € (plus VAT)

 

Participants

Max. 3

 

For additional information please send a request to courses .microscopy .de @zeiss .com.

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