Fluorescence Correlation Spectroscopy (FCS) @ LSM 980

Fluorescence Correlation Spectroscopy (FCS) is a highly sensitive method that detects movement and binding of fluorescence-labeled particles on the single molecule level. Furthermore, concentrations of movable fluorescent particles can be estimated. Thus biochemical reactions can be monitored in real time solutions as well as within living cells.
This course will familiarize you with the theoretical background of FCS and train you on how to perform and evaluate measurements in solutions.

Topics of the course

 

  • Theory of FCS
  • Correlation function
  • Model functions for diffusion processes
  • Data acquisition for FCS and FCCS
  • Data analysis for FCS and FCCS
  • Objective lenses for FCS and FCCS
  • Short introduction to RICS

 

Learning Level

Expert

 

Prerequisites

Profound knowledge of LSM 980 is recommended

 

Systems

LSM 980

 

Software

ZEN (blue edition)

 

Date

March 1 to 2, 2023 
Registration until 2 weeks prior to the training

 

Duration

2 days

 

Location

Training and Support Center Jena

 

Language

English

 

Course Fee

Per Person: 1440 € (plus VAT)

 

Participants

Max. 3

 

For additional information please send a request to courses .microscopy .de @zeiss .com.

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